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Study accumulation of secondary metabolites in plants
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USER GUIDE FOR REMOTE VIEW OF RTV-5 EXPERIMENTAL ANALYSIS

 

 

1.Open Internet Explorer/Google chrome/Mozilla Firefox, preferably Internet Explorer.

 

2.Type XXXXXXXXXX on the address bar (ID will be provided verbally) (Please contact padmavati@gmail.com for the public access to the IP).

 

3.A window page will be opened displaying the Shimadzu CBM 20A HPLC explorer.

 

4.On the left side of the window, a menu option is available.

 

5.Click on L20224913000 option.

 

6.On the top of right side of the window page, click on the system name (Click to login) option with the link

L20224913000.

 

7.Login screen will be displayed requesting for user id and password.

 

8.After entering the login details the users will be able to visualize the window page displaying Configuration, Analysis, Editing and Queue options.

 

9.Configuration option provide details about the instrument model, version, source of connection, mode of gradientused for elution of compounds i.e. binary or isocratic and lamp used for detector.

 

10.Analysis option is used to visualize the various parameters required for operating the HPLC column such as pump, gradient, wavelength, flow rate etc.

 

11.Clicking on editing option allows setting various parameters regarding the chromatographic run.

 

12.Then click on individual parameters displayed such as pump, detector, time program and gradient.

 

13.Individual windows of the functions mentioned above will be opened in which the users can input the desired values for running the column.

 

14.Then click on “LC time program” button provided in the right side corner of the bottom of the page.

 

15.Users can set time (minutes), module, function (pump B) and value (% of Solvent B altered according to time).Then click on ok button to save the time program which will be maintained during the entire run of the gradient.

 

16.After entering all the values for given function, click on ok button and save the file on your PC.

 

17.Then click on the option “Load from PC”

 

18.Choose the method file created for the gradient run saved on your PC.

 

19.Upload the ‘saved method file’ and press the pump button to activate the flow of the mobile phase.

 

20.After stabilizing the gradient for few minutes, the sample should be injected manually in the sample injector.

 

21.Finally the ready option will be changed to run option once the users will click on “Run”.

 

22.The separation of the compounds in the injected sample will be achieved and will be observed in the chromatogram.

 

NOTE:

 

1. One user at a time can be allowed for a remote view when analysis parameters need to be changed.


2. Multiple users may be allowed for a remote view when analysis parameters are fixed and the analysis is either in demo mode/running mode. 

 

 

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REMOTE TESTING OF PLANT METABOLIC PATHWAY LABORATORY

 

  • The laboratory sessions were divided into two sessions as mentioned above in the text.

 

  • Before starting the classes all  internetconnections were checked thoroughly in the PC’s.

 

  • Before getting into the experimental details, students were provided with a lecture regarding the significance of a virtual laboratory as an educational tool and its utilization with respect to secondary metabolism.

 

  • For remote linking of the HPLC instrument a step by step protocol were provided for all the students.

 

  • The protocol describes about functioning of the instrument and setting of various parameters from remote location.

 

  • One of the most important criteria is that one person is always with the instrument in the actual lab and monitors all the changes made by the users from outside when they are connected. The person has to inject a sample in the injector of the HPLC instrument which is a manual process.

 

  • After injecting the sample, the user from outside location  will be able to visualize the chromatogram of the HPLC in their PC’s window.

 

  • This process enables students to observe the results andthey may request for result which may be send for further use.  

 

 

Number of Educational Institutes visited- Sri Venkateswara University, Tirupati; Jadavpur University, Kolkata;

 

Bidhan Chandra Krishi Viswavidyalaya, Nadia; KIIT University, Bhubaneswar;  National Institute of Technology,

 

Rourkela; IITBhubaneswar, Bhubaneswar; National Botanical Research Institute, Lucknow.  

 

 

Period of visit- March 2013- May 2014

 

 

 

Table 1: Remote Testing of Plant Metabolic Pathway Laboratory 

 

 

 

Figure 1: Learning outcomes obtained from a Plant Metabolic Pathway Virtual Laboratory Demonstration

 

 

 

User feedback: The feedback obtained from the field study is incorporated accordingly and ongoing. 

 

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