. .
Demonstrate the extraction and qualitative and quantitative estimation of secondary metabolites





1. Handbook of Food and Analytical Chemistry-Pigments, Colorants, Flavors, Texture and Bioactive Food Components Vol-2. Ronald E.Wrolstad, Terry E.Acree, Eric A.Decker, Michael H.Penner, David S. Reid, Steven J. Schwartz, Charles F. Shoemaker, Denise Smith and Peter Sporns. John Wiley & Sons, Inc Publication 2000-2005.


2.  Phenolic Compound Biochemistry. Wilferd Vermerris and Ralph Nicholson. Springer Science +Business Media B.V. 2008.


3.   Methods in Polyphenol analysis. C.Santos-Buelga and G.Williamson. Royal Society of Chemistry; 2003.


4.  Extraction Technologies for Medicinal and Aromatic plants. Sukhdev Swami Handa, Suman Preet Singh Khanuja,Gennaro Longo, Dev Dutt Rakesh. International Centre for Science and High Technology, Trieste, 2008.


5.   Tea: The Plant and its Manufacture; Chemistry and Consumption of the Beverage. Douglas A. Balentine, Matthew E.Harbowy and Harold N. Graham. In: Caffeine, 1997; pp-1-38.


6.   PETUNIA, Petunia x hybrida. Robert J.Griesbach. In: Flower Breeding   and Genetics, 2006; pp-301-336.


7.  The Genetics of Flower Color. G.Tornielli, R.Koes, F.Quattrocchio. In: Petunia-Evolutionary, Developmental and Physiological Genetics, 2009; pp-269-299.


8.  Petunia Biotechnology. Beverly A.Underwood, Michelle L.Jones and David G.Clerk. In: Petunia- Evolutionary, Developmental and Physiological Genetics, 2009; pp-411-433.


9.  An efficient method for the extraction of polyphenolics from some traditional   varieties of North-east India. A. Begum, A. Goswami, P.K. Goswami and P.Chowdhary. In: Chemistry of Phytopotentials: Health Energy and Environmental Perspectives, 2012; pp-285-288.







1.  Vargas, F.D. et al., (2000). Natural pigments: Carotenoids, Anthocyanins and Betalins- Characterstics, Biosynthesis, Processing and Stability. Critical Reviews in Food Science and Nutrition, Vol.40, No.3, pp-173-289.


2.  Dmitrienko, S.G. et al., (2012). Methods of extraction, Preconcentration and Determination of Quercetin. Journal of Analytical Chemistry, Vol.67, No.4, pp-299-311.


3.  Stalikas, C.D. (2007).Extraction, Separation and detection methods for phenolic acids and flavonoids. Journal of Separation Science, Vol.30, pp-3268-3295. 


4.  Forrest, G.I. and Bendall,D.S.(1969). The Distribution of polyphenols in the Tea plant (Camellia sinensis L.).Biochemical journal, Vol.113, pp-741-755.


5.  Hirun, S. and Roach, P.D. (2011).An improved solvent extraction method for the analysis of catechins and caffeine in green tea. Journal of Food and Nutrition Research, vol.50, No.3, pp-160-166.


6.  Sakakibara, S. et al., (2003).Simultaneous Determination of All Polyphenols in Vegetables, Fruits and Teas. Journal of Agricultural and Food Chemistry, vol.51, pp-571-581.


7.  Dalluge, J.J. et al., (1993).Selection of column and gradient solution system for the separation of catechins in green tea using high performance liquid chromatography. Journal of Chromatography A, Vol.793, pp-265-274.


8.  Goto, et al., (1996b). Simultaneous analysis of individual catechins and caffeine in green tea. Journal of Chromatography A, Vol.749, pp-295-299.


9.  Bronner, W.E. and Beecher, G.R. (1998). Method for determining the content of catechins in tea infusions by HPLC. Journal of Chromatography A, Vol.805, pp-137-142.























Cite this Simulator:

..... .....
Copyright @ 2018 Under the NME ICT initiative of MHRD (Licensing Terms)
 Powered by AmritaVirtual Lab Collaborative Platform [ Ver 00.12. ]